DPPH repair the damage caused by the free radicals.

 

DPPH Radical Scavenging Activity

DPPH is a stable free radical which has the
tendency to accept electrons to become stable molecules. Basically, it is used
to screen the antioxidant activity of various samples. DPPH radical can be
measured at absorbance maxima at 517 nm. Decrease in the absorbance indicates
the acceptance of electrons which is induced by the antioxidants. The results
are shown in Figure 1  ethanolic extracts
of Celery leaves has IC 50 of  mg/ml.The
antioxidant activity of the extracts is credited to their hydrogen donating
ability (Yamaguchi et al., 1998). Results suggest extracts have strong
potential in scavenging the free radical, which could be attributable to its
hydrogen donating ability.

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Superoxide Radical Scavenging

Inhibitory effects of aqueous and ethanolic
extracts of Celery on superoxide radicals are shown in Figure 2. Scavenging of
superoxide radicals with IC50 of 1.7 was observed in aqueous and ethanolic
extracts. Superoxide radicals are generated during the normal physiological
process, mainly in mitochondria. It is well-known that superoxide anion is a
weak oxidant and further undergoes oxidation to give hydroxyl radical and
singlet oxygen, which are harmful, leading to oxidative stress (Dahl and Richardson,
1978; and Meir et al., 1995). Therefore, superoxide radical scavenging by antioxidants
has physiological implications.

 

Lipid Peroxidation

Inhibition of lipid peroxidation was
observed ethanolic extracts of Celery with an IC50 of  and (Figure 3). Oxidation of polyunsaturated
fatty acids in the cell membrane produces Malondialdehyde (MDA), which is the
index of lipid peroxidation and marker of cell injury. Cell damage can occur in
any internal organ by free radicals, leading to various disorders, viz.,
atherosclerosis, hepato and nephro damage (Janero, 1990; and Rice-Evans and
Burdon, 1993). Our results showed inhibition of lipid peroxidation with
increase in concentration of the extracts, indicating that both aqueous and
ethanolic extracts have certain antioxidant molecules which are able to repair
the damage caused by the free radicals. The mechanism in inhibiting the lipid peroxidation
by the extracts could be by preventing the chain initiation of polyunsaturated fatty
acid chain by donating the hydrogen atom to the damaged lipid bilayer.

 

Reducing Power

The reducing power of the extracts and BHA
values are shown in Figure 4. The extracts showed increasing activity with
increase in the concentration of extracts. BHA was more potent, with a minimum
concentration of mg showing highest reducing power compared to the extracts. Reducing
capacity of the extracts could be considered an indicator towards its potential
antioxidant properties (Meir et al., 1995). Total potential of the antioxidant activity
has been attributed to various mechanisms viz., prevention of chain initiation,
binding of transition metal ion, inhibition of hydrogen abstraction, radical
scavenging and preventing lipid bilayer damage (Diplock, 1997; and Yildirim et
al., 2001). Duh (1998) has shown that reducing properties are associated with
the presence of reductones. Reductones play a major role in exerting
antioxidant action by donating hydrogen atom and preventing the free radical
chain damage. It also reacts with precursors of peroxide and prevents the
peroxide formation (Gordon, 1990; and Yen and Chen, 1995). Results suggest that
both the extracts of Celery  extract have
potential to free radical damage by donating hydrogen atom thereby preventing
oxidative stress.

 

Phenolic Content

Phenolic content in the aqueous extract of Celery
of the ethanolic extract (18.20±5.4 and 12.96±3.7 mg guaicol equivalent per gram,
respectively). Phenols play a major role in radical scavenging because of their
hydroxyl groups (Hatano et al., 1989). Total phenolic content present in the
extract is directly related to antioxidant activity (Duh et al. , 1999). It is
reported that polyphenolic compounds protect humans from mutagenesis and
carcinogenesis (Tanaka et al. , 1998). In our study, there is a correlation
between antioxidant activity and phenol content. The various antioxidant
activity of aqueous and ethanolic extracts of Celery demonstrated in this study
clearly indicates the potential application value of Celery.

 

 

Cytoprotection of Celery on yeast cells against CCl4 induced
damage

 

In vitro cell culture play a model framework
in comprehension the role of the phytochemical in

ameliorating the level of oxidative stress
induced by xenobiotic in cells, which is measured by cell viabilityA few
studies on photochemical have indicated cytoprotective impact in both in
vitro and in vivo models 8. In this test we have demonstrated the celery
extract in anticipating xenobiotic induced cell death in yeast cells. CCl4  inducer of oxidative stress in cells is
utilized as toxicant. Our outcomes indicated restraint of xenobiotic induced
lipid peroxidation, inhibition of ROS by preventing cell death and reduction in
level of LDL leakage with increase in concentration of extract. Cytoprotection
observed as cell viability, was observed for cells co treatment with
100–500?g/ml of extract and 0.01mM endosulfan Fig.1. LDH leakage in the cells
was altogether decreased when cells co treated with increasing concentration of
extract, compared with CCl4 treated Fig.2. Lipid peroxidation was depleted in
the cells treated with high convergence of extracts, in which the development
of Malondialdehyde was measured as marker record of lipid bilayer damage Fig 3.
Reactive oxygen species (ROS), level increases when cells exposed to stress
condition. The level of ROS was reduced when cells co treated with the extracts
Fig 4. These outcomes demonstrate that the unrefined extract might contain
cocktail of photochemicals, which improve the level of oxidative stress
instigated by the CCl4 by protecting the cell from undergoing death. Further
extract needs to investigate the photochemical in charge of keeping the cell
alive.